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N. Rahmah, B.H. Lim, A.Khairul Anuar, R.K. Shenoy, V. Kumaraswami, S.Lokman Hakim, P. Chotechuang, K. Kanjanopas, C.P. Ramachandran, A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection, Transactions of The Royal Society of Tropical Medicine and Hygiene, Volume 95, Issue 3, May-June 2001, Pages 280–284, https://doi.org/10.1016/S0035-9203(01)90234-2
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Abstract
An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0.300, the IgG4 ELISA demonstrated specificity rates of 95·6–100%, sensitivity rates of 96–100%, positive predictive values of 75–100% and negative predictive values of 98·9–100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis.
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